Fluorometric determination of ammonia with fluorescamine
نویسندگان
چکیده
منابع مشابه
Total fluorometric amino acid analysis using fluorescamine.
A fllwrometric amino acid analyzer using fllmresramine for the assay of the full array of natural amino ac<ids including prolinc on a single column is reported. The proline determination was carried out by specific introduction of a solution of ,\‘chlorosl~ccinimide into the flow system. Single rolumn fluorometric amino acid analysis was curried out in a significantly shorter time and with a se...
متن کاملFluorometric Quantitation of Protein using the Reactive Compound Fluorescamine
Total protein content is a measurement common to many applications in basic science and clinical research. Several different fluorescent techniques are available that eliminate many of the problems associated with the traditional absorbance-based colorimetric methods to measure total protein content. Here we describe the use of fluorescamine to quantitate total protein using the BioTek FL600 fl...
متن کاملDetermination of long-chain base in glycosphingolipids with fluorescamine.
A method is described for determining the long-chain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.
متن کاملFluorometric determination of histidine.
A. FLUOROMETRIC METHOD for the determination of histidine was developed in response to the need for an accurate and precise manual method for the confirmation of histidinemia (1). it is based on the fluorometric method of Shore et a!. (2), which involves the condensation of histaniine with o-phthalaidehyde (OPT) in a strongly alkaline solution to form a rather labile fluorescent compound which ...
متن کاملFluorometric determination of propoxyphene.
With 4-chloro-7-nitrobenzo-2, 1,3-oxadiazole (I), fluorescent derivatives of propoxyphene can be prepared, thus enabling quantitation of the drug in biological samples. This technique offers an acceptable alternative to gas chromatographic (1-5) or ultraviolet spectrophotometric (6, 7) procedures. Favorable aspects of the fluorometric technique are high sensitivity, specificity, potential for r...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1981
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.30.2_136